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ftsX [2018-05-04 18:19:41]
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ftsX [2018-05-04 18:19:41]

ABC transporter (membrane protein) (FtsE-FtsX), required for CwlO activity (cell elongation) and for proper activation of Spo0A and initiation of sporulation
locus
BSU35250
pI
10.02
mw
32.95 kDa
protein length
296 aa Sequence Blast
gene length
888 bp Sequence Blast
function
control of cell wall synthesis
product
ABC transporter (membrane protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.2|Autolytic activity required for peptidoglycan synthesis (cell elongation)]
  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.3|Regulatory ABC transporters]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,623,938 → 3,624,828

    Phenotypes of a mutant

  • a ''[gene|E22D0F0AC56DC95AFC02E7F55FCD5FAA44B6B490|ftsX]'' mutation is synthetically lethal with a ''[gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE]'' mutation (due to a lack of autolysin activity) [Pubmed|23869552,23855774]
  • shorter, fatter cells, this can be rescued by addition of Mg(2+) [Pubmed|23869552,23855774]
  • reduced growth rate, especially at low Mg(2+) concentrations [Pubmed|23869552]
  • loss of genetic competence [pubmed|29553055]

    • The protein

    Catalyzed reaction/ biological activity

  • necessary for the autolysin activity of [protein|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|CwlO] [Pubmed|23855774]

    • Protein family

  • FtsX subfamily (according to Swiss-Prot)

    • [SW|Localization]

  • cell membrane [Pubmed|23869552]

    • Expression and Regulation

    Operons

    genes
    [gene|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|ftsE]-[gene|E22D0F0AC56DC95AFC02E7F55FCD5FAA44B6B490|ftsX]
    description
    (according to [http://dbtbs.hgc.jp/COG/prom/ftsEX.html DBTBS]) null

    regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

    • regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
    • view in new tab

    Biological materials

    Mutant

  • BKE35250 (Δ[gene|E22D0F0AC56DC95AFC02E7F55FCD5FAA44B6B490|ftsX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCACGCAAGTGGCGCCCGA, downstream forward: _UP4_TAAAGTGAAAAAGCCGTTCC
  • BKK35250 (Δ[gene|E22D0F0AC56DC95AFC02E7F55FCD5FAA44B6B490|ftsX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCACGCAAGTGGCGCCCGA, downstream forward: _UP4_TAAAGTGAAAAAGCCGTTCC

    • References

  • 10092453,18573177,23651456,14651647,18763711,23855774,23869552,29553055